Ectopic expression of an FT homolog from Citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.)
Identifieur interne : 002849 ( Main/Exploration ); précédent : 002848; suivant : 002850Ectopic expression of an FT homolog from Citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.)
Auteurs : Tomoko Endo [Japon] ; Takehiko Shimada [Japon] ; Hiroshi Fujii [Japon] ; Yasushi Kobayashi [Japon] ; Takashi Araki [Japon] ; Mitsuo Omura [Japon]Source :
- Transgenic research [ 0962-8819 ] ; 2005.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Citrus FT (CiFT) cDNA, which promoted the transition from the vegetative to the reproductive phase in Arabidopsis thaliana, when constitutively expressed was introduced into trifoliate orange (Poncirus trifoliata L. Raf.). The transgenic plants in which CiFT was expressed constitutively showed early flowering, fruiting, and characteristic morphological changes. They started to flower as early as 12 weeks after transfer to a greenhouse, whereas wild-type plants usually have a long juvenile period of several years. Most of the transgenic flowers developed on leafy inflorescences, apparently in place of thorns; however, wild-type adult trifoliate orange usually develops solitary flowers in the axils of leaves. All of the transgenic lines accumulated CiFT mRNA in their shoots, but there were variations in the accumulation level. The transgenic lines showed variation in phenotypes, such as time to first flowering and tree shape. In F1 progeny obtained by crossing 'Kiyomi' tangor (C. unshiu x sinensis) with the pollen of one transgenic line, extremely early flowering immediately after germination was observed. The transgene segregated in F1 progeny in a Mendelian fashion, with complete co-segregation of the transgene and the early flowering phenotype. These results showed that constitutive expression of CiFT can reduce the generation time in trifoliate orange.
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Raf.)</title><author><name sortKey="Endo, Tomoko" sort="Endo, Tomoko" uniqKey="Endo T" first="Tomoko" last="Endo">Tomoko Endo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Citrus Research, National Institute of Fruit Tree Science, National Agricultural Research Organization</s1><s2>Okitsu Shimizu-ku, Shizuoka, 424-0292</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Okitsu Shimizu-ku, Shizuoka, 424-0292</wicri:noRegion></affiliation></author><author><name sortKey="Shimada, Takehiko" sort="Shimada, Takehiko" uniqKey="Shimada T" first="Takehiko" last="Shimada">Takehiko Shimada</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Citrus Research, National Institute of Fruit Tree Science, National Agricultural Research Organization</s1><s2>Okitsu Shimizu-ku, Shizuoka, 424-0292</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Okitsu Shimizu-ku, Shizuoka, 424-0292</wicri:noRegion></affiliation></author><author><name sortKey="Fujii, Hiroshi" sort="Fujii, Hiroshi" uniqKey="Fujii H" first="Hiroshi" last="Fujii">Hiroshi Fujii</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Citrus Research, National Institute of Fruit Tree Science, National Agricultural Research Organization</s1><s2>Okitsu Shimizu-ku, Shizuoka, 424-0292</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Okitsu Shimizu-ku, Shizuoka, 424-0292</wicri:noRegion></affiliation></author><author><name sortKey="Kobayashi, Yasushi" sort="Kobayashi, Yasushi" uniqKey="Kobayashi Y" first="Yasushi" last="Kobayashi">Yasushi Kobayashi</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Botany, Graduate School of Science, Kyoto University</s1><s2>Sakyo-ku, Kyoto, 606-8502</s2><s3>JPN</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName><orgName type="university">Université de Kyoto</orgName></affiliation></author><author><name sortKey="Araki, Takashi" sort="Araki, Takashi" uniqKey="Araki T" first="Takashi" last="Araki">Takashi Araki</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Botany, Graduate School of Science, Kyoto University</s1><s2>Sakyo-ku, Kyoto, 606-8502</s2><s3>JPN</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName><orgName type="university">Université de Kyoto</orgName></affiliation></author><author><name sortKey="Omura, Mitsuo" sort="Omura, Mitsuo" uniqKey="Omura M" first="Mitsuo" last="Omura">Mitsuo Omura</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Citrus Research, National Institute of Fruit Tree Science, National Agricultural Research Organization</s1><s2>Okitsu Shimizu-ku, Shizuoka, 424-0292</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Okitsu Shimizu-ku, Shizuoka, 424-0292</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">06-0028036</idno><date when="2005">2005</date><idno type="stanalyst">PASCAL 06-0028036 INIST</idno><idno type="RBID">Pascal:06-0028036</idno><idno type="wicri:Area/PascalFrancis/Corpus">000595</idno><idno type="wicri:Area/PascalFrancis/Curation">000409</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000621</idno><idno type="wicri:doubleKey">0962-8819:2005:Endo T:ectopic:expression:of</idno><idno type="wicri:Area/Main/Merge">002977</idno><idno type="wicri:Area/Main/Curation">002849</idno><idno type="wicri:Area/Main/Exploration">002849</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Ectopic expression of an FT homolog from Citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.)</title><author><name sortKey="Endo, Tomoko" sort="Endo, Tomoko" uniqKey="Endo T" first="Tomoko" last="Endo">Tomoko Endo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Citrus Research, National Institute of Fruit Tree Science, National Agricultural Research Organization</s1><s2>Okitsu Shimizu-ku, Shizuoka, 424-0292</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Okitsu Shimizu-ku, Shizuoka, 424-0292</wicri:noRegion></affiliation></author><author><name sortKey="Shimada, Takehiko" sort="Shimada, Takehiko" uniqKey="Shimada T" first="Takehiko" last="Shimada">Takehiko Shimada</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Citrus Research, National Institute of Fruit Tree Science, National Agricultural Research Organization</s1><s2>Okitsu Shimizu-ku, Shizuoka, 424-0292</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Okitsu Shimizu-ku, Shizuoka, 424-0292</wicri:noRegion></affiliation></author><author><name sortKey="Fujii, Hiroshi" sort="Fujii, Hiroshi" uniqKey="Fujii H" first="Hiroshi" last="Fujii">Hiroshi Fujii</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Citrus Research, National Institute of Fruit Tree Science, National Agricultural Research Organization</s1><s2>Okitsu Shimizu-ku, Shizuoka, 424-0292</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Okitsu Shimizu-ku, Shizuoka, 424-0292</wicri:noRegion></affiliation></author><author><name sortKey="Kobayashi, Yasushi" sort="Kobayashi, Yasushi" uniqKey="Kobayashi Y" first="Yasushi" last="Kobayashi">Yasushi Kobayashi</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Botany, Graduate School of Science, Kyoto University</s1><s2>Sakyo-ku, Kyoto, 606-8502</s2><s3>JPN</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName><orgName type="university">Université de Kyoto</orgName></affiliation></author><author><name sortKey="Araki, Takashi" sort="Araki, Takashi" uniqKey="Araki T" first="Takashi" last="Araki">Takashi Araki</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Botany, Graduate School of Science, Kyoto University</s1><s2>Sakyo-ku, Kyoto, 606-8502</s2><s3>JPN</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName><orgName type="university">Université de Kyoto</orgName></affiliation></author><author><name sortKey="Omura, Mitsuo" sort="Omura, Mitsuo" uniqKey="Omura M" first="Mitsuo" last="Omura">Mitsuo Omura</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Citrus Research, National Institute of Fruit Tree Science, National Agricultural Research Organization</s1><s2>Okitsu Shimizu-ku, Shizuoka, 424-0292</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Okitsu Shimizu-ku, Shizuoka, 424-0292</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Transgenic research</title><title level="j" type="abbreviated">Transgenic res.</title><idno type="ISSN">0962-8819</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Transgenic research</title><title level="j" type="abbreviated">Transgenic res.</title><idno type="ISSN">0962-8819</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus</term><term>Flowering</term><term>Phenotype</term><term>Poncirus trifoliata</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Floraison</term><term>Phénotype</term><term>Citrus</term><term>Poncirus trifoliata</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Citrus FT (CiFT) cDNA, which promoted the transition from the vegetative to the reproductive phase in Arabidopsis thaliana, when constitutively expressed was introduced into trifoliate orange (Poncirus trifoliata L. Raf.). The transgenic plants in which CiFT was expressed constitutively showed early flowering, fruiting, and characteristic morphological changes. They started to flower as early as 12 weeks after transfer to a greenhouse, whereas wild-type plants usually have a long juvenile period of several years. Most of the transgenic flowers developed on leafy inflorescences, apparently in place of thorns; however, wild-type adult trifoliate orange usually develops solitary flowers in the axils of leaves. All of the transgenic lines accumulated CiFT mRNA in their shoots, but there were variations in the accumulation level. The transgenic lines showed variation in phenotypes, such as time to first flowering and tree shape. In F<sub>1</sub> progeny obtained by crossing 'Kiyomi' tangor (C. unshiu x sinensis) with the pollen of one transgenic line, extremely early flowering immediately after germination was observed. The transgene segregated in F<sub>1</sub> progeny in a Mendelian fashion, with complete co-segregation of the transgene and the early flowering phenotype. These results showed that constitutive expression of CiFT can reduce the generation time in trifoliate orange.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région du Kansai</li></region><settlement><li>Kyoto</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><country name="Japon"><noRegion><name sortKey="Endo, Tomoko" sort="Endo, Tomoko" uniqKey="Endo T" first="Tomoko" last="Endo">Tomoko Endo</name></noRegion><name sortKey="Araki, Takashi" sort="Araki, Takashi" uniqKey="Araki T" first="Takashi" last="Araki">Takashi Araki</name><name sortKey="Fujii, Hiroshi" sort="Fujii, Hiroshi" uniqKey="Fujii H" first="Hiroshi" last="Fujii">Hiroshi Fujii</name><name sortKey="Kobayashi, Yasushi" sort="Kobayashi, Yasushi" uniqKey="Kobayashi Y" first="Yasushi" last="Kobayashi">Yasushi Kobayashi</name><name sortKey="Omura, Mitsuo" sort="Omura, Mitsuo" uniqKey="Omura M" first="Mitsuo" last="Omura">Mitsuo Omura</name><name sortKey="Shimada, Takehiko" sort="Shimada, Takehiko" uniqKey="Shimada T" first="Takehiko" last="Shimada">Takehiko Shimada</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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